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Image Search Results
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology
Article Title: Improvement of detection specificity of Plasmodium-infected murine erythrocytes by flow cytometry using autofluorescence and YOYO-1.
doi: 10.1002/cyto.a.20169
Figure Lengend Snippet: FIG. 5. Characterisation of CD711 reticulocytes infected by Plasmo- dium yoelii by FL-2/YOYO-1 analysis. A, B: Expression of CD71 in ery- throcytes from noninfected control mice. Samples from noninfected con- trols were fixed and stained with YOYO-1 as described. Subsequently these samples were stained with biotin-isotype matched-control or biotin- CD71 monoclonal antibody. Erythrocytes were gated on logarithmic side/ scatter plots. Data are from a representative individual out of five. C–E: Separation of CD711 reticulocytes of peripheral blood from CD1 mice infected with P. yoelii. A sample of peripheral blood from a CD1 mouse (3% of parasitemia) was taken 48 h after infection with 6.4 3 106 P. yoe- lii–infected erythrocytes. After separation according to the expression of CD71 (see Materials and Methods), the positive (CD711) and negative (CD712) fractions were stained with YOYO-1 and analyzed in FL-2/YOYO- 1 bidimensional analysis. Before separation, the percentage of CD711
Article Snippet: Pathogen-free
Techniques: Infection, Expressing, Control, Staining